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KMID : 0376219830200020247
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Chonnam Medical Journal
1983 Volume.20 No. 2 p.247 ~ p.256
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Recovery grade of the properties of streptomycin resistant bacteria-passed phage by passing through normal bacteria
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朴寶漢/Park, Bohan
李洪洙/辛富安/Lee, Hong Su/Shin, Boo-Ahn
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Abstract
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The recovery grade, by passing through normal bacteria, of properties of the phage passed three times through streptomycin(SM) resistant bacteria was investigated. For this experiment, the Klebsiella phage(Kp phage), it´s host cell, Klebsiella pneumoniae, type 2, (Kp) and SM resistant Kp(SMKp) were used. The following results were obtained.
The adsorptions of the phage passed three times through SMKp(SMKp-phage) to SMKp and Kp were similar to that of Kp phage.
If the SMKp-phage is passed three times through Kp, the plaque appearance in SMKp became more similar to that of Kp phage than SMKp-phage. However, in Kp, the SMKp-phage, Kp-passed SMKp-phage and Kp phage formed analogous plaques.
The halo plaque formation of the SMKp-phage became similar grade, against SMKp-phage, to that of Kp phage by passing three times through Kp.
The phage growth in SMKp and Kp was still similar, against Kp phage, to that of SMKp-phage even though the SMKp-phage is passed 16 times through Kp.
The antigenicity of the SMKp-phage passed 19 times or 21 times through Kp was much different from that of Kp phage against the SMKp-phage.
When the Kp passed, 21 times, SMKp-phage, SMKp-phage and Kp phage are neutralized by lower concentration of the anti-Kp phage serum, the initial lag in the reaction was appeared and it showed egually a theoretical two-hit curve appearance.
Both recoveries of the SMKp-phage from SMKp and Kp were similar as that of Kp phage.
The minimum inhibitory concentration of SM to the SMKp and Kp was 140 mg/ml and 0.02 mg/ml.
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